Composite

Part:BBa_I716273:Design

Designed by: Vaibhavi Umesh   Group: iGEM07_Berkeley_UC   (2007-10-25)


T7 promoter (I716105) + [1090 + SodC gene (I716257)]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 592
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 607
    Illegal AgeI site found at 361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1694


Design Notes

none


Source

Composite part of T7 promoter (I716105) + [1090 + SodC gene (I716257)]

Digest T7 promoter (I716105) (EcoRI/BamHI,,L) Sub into [1090 rbs + sodC gene (I716257)] (EcoRI/BglII, 2610 + 9, L) Product is I716273


References